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KMID : 0359320210610020013
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Korean Journal of Veterinary Research
2021 Volume.61 No. 2 p.13 ~ p.13
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Isolation and identification of mammalian orthoreovirus type 3 from a Korean roe deer (Capreolus pygargus)
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Yang Dong-Kun
An Sung-Jun
Park Ye-Seul
Yoo Jae-Young
Park Yu-Ri
Park Jung-Won
Kim Jong-Taek
Ahn Sang-Jin
Hyun Bang-Hun
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Abstract
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Mammalian reovirus (MRV) causes respiratory and intestinal disease in mammals. Although MRV isolates have been reported to circulate in several animals, there are no reports on Korean MRV isolates from wildlife. We investigated the biological and molecular characteristics of Korean MRV isolates based on the nucleotide sequence of the segment 1 gene. In total, 144 swabs from wild animals were prepared for virus isolation. Based on virus isolation with specific cytopathic effects, indirect fluorescence assays, electron microscopy, and reverse transcription-polymerase chain reaction, only one isolate was confirmed to be MRV from a Korean roe deer (Capreolus pygargus). The isolate exhibited a hemagglutination activity level of 16 units with pig erythrocytes and had a maximum viral titer of 105.7 50% tissue culture infectious dose (TCID50)/mL in Vero cells at 5 days after inoculation. The nucleotide and amino-acid sequences of the partial segment S1 of the MReo2045 isolate were determined and compared with those of other MRV strains. The MReo2045 isolate had nucleotide sequences similar to MRV-3 and was most similar (96.1%) to the T3/Bat/Germany/342/08 strain, which was isolated in Germany in 2008. The MReo2045 isolate will be useful as an antigen for sero-epidemiological studies and developing diagnostic tools.
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KEYWORD
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reovirus, virus isolation, identification, genetic characterization, roe deer
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